Porphyromonas gingivalis 분쇄액으로 유도된 파골세포의 분화에 미치는 Taurine과 Alendronate의 효과
THE INHIBITORY EFFECT OF TAURINE AND ALENDRONATE ON THE OSTEOCLAST DIFFERENTIATION MEDIATED BY SONICATED EXTRACTS OF PORPHYROMONAS GINGIVALIS IN VITRO
박주현, 금기연, 이정현, 유윤정, 이승종,
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박주현 ( Park Ju-Hyun ) - 연세대학교 치과대학 치과보존학교실
금기연 ( Kum Kee-Yeon ) - 연세대학교 치과대학 치과보존학교실
이정현 ( Lee Jung-Hyun ) - 연세대학교 치과대학 구강생물학교실
유윤정 ( Yu Yoon-Jeong ) - 연세대학교 치과대학 구강생물학교실
이승종 ( Lee Seung-Jong ) - 연세대학교 치과대학 치과보존학교실
KMID : 0362320010260040285
Abstract
The objective of this study was to investigate the inhibitory effect of taurine and alendronate on the osteoclast differentiation. Osteoblasts and bone marrow cells from 1-2 day old mouse were co-cultured in 10% fetal bovine serum - minimal essential media (FBS-MEM). Osteoclast differentiation was induced by adding the sonicated extracts of Porphyromonas gingivalis (P.gingivalis). Osteoclasts were identified using tartrate resistant acid phosphotase staining (TRAP). Alendronate of 10-7, 10-6, 10-5M and taurine of 500, 1000, 1500μg/ml were added respectively. The cytotoxic effects of alendronate and taurine were examined using MTT(3-(4,5-dimethylthiazol -2-yl-2,5 -diphenyltetrazo- lium bromide) method. After culturing with the sonicated extracts of P. gingivalis, the amounts of IL-6 in the culture supernatants were measured and compared using the ELISA method. The results were as follows:
1. Osteoclasts were differentiated at the concentration of 0.01??0.1μg/ml sonicated extracts of P.gingivalis. (P<0.05)
2. Alendronate inhibited osteoclasts differentiation at the concentration of 10-5 M when the concentration of sonicated extracts of P.gingivalis was 0.01μg/ml.
3. Taurine inhibited osteoclasts differentiation at the concentration of 1500μg/ml when the concentration of sonicated extracts of P.gingivalis was 0.01μg/ml.
4. In cytotoxic test (MTT test), no cytotoxic effect was evident in all concentrations of alendronate and taurine.
5. Taurine(10-5M) and alendronate(1500μg/ml) did not change the amounts of IL-6 induced by sonicated extracts of P. gingivalis significantly.
키워드
Alendronate ; Taurine ; P.gingivalis ; Osteoclast ; Replacement root resorption
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